オミクロン感染後ウイルス培養が陽性となる期間は約8日

コロラド先生のTLで見かけたツイート

つまり、症状が出るかPCR検査で偶々陽性となってから8日前後、だいたい1週間から10日ほどは、たとえ感染者本人がピンピンしてても他人に移すほど大量のウイルスを排出し続ける能力を保持してるので、他人に迷惑をかけたくなければ大人しくしててね、いうことなのですが・・・

元ネタの論文を見るとタイトルに[BA.1]と付いていてまして、いわゆる初期オミクロン株のことです。すでに日本にも侵入していて今後一気に感染拡大するであろう凶暴な変異株[BA.5]は、武漢株と初期オミクロン[BA.1]のかけ離れ方と同じくらい、[BA.1]とかけ離れた代物だとされています。なので、今まで以上に要注意。

 

Duration of Shedding of Culturable Virus in SARS-CoV-2 Omicron (BA.1) Infection
【NEJM 2022年6月29日】

The B.1.1.529 (omicron) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has a shorter incubation period and a higher transmission rate than previous variants.1,2 Recently, the Centers for Disease Control and Prevention recommended shortening the strict isolation period for infected persons in non–health care settings from 10 days to 5 days after symptom onset or after the initial positive test, followed by 5 days of masking.3 However, the viral decay kinetics of the omicron variant and the duration of shedding of culturable virus have not been well characterized.

We used longitudinal sampling of nasal swabs for determination of viral load, sequencing, and viral culture in outpatients with newly diagnosed coronavirus disease 2019 (Covid-19).4 From July 2021 through January 2022, we enrolled 66 participants, including 32 with samples that were sequenced and identified as the B.1.617.2 (delta) variant and 34 with samples that were sequenced and identified as the omicron subvariant BA.1, inclusive of sublineages. Participants who received Covid-19–specific therapies were excluded; all but 1 participant had symptomatic infection. This study was approved by the institutional review board and the institutional biosafety committee at Mass General Brigham, and informed consent was obtained from all the participants.

The characteristics of the participants were similar in the two variant groups except that more participants with omicron infection had received a booster vaccine than had those with delta infection (35% vs. 3%) (Tables S1 and S2 in the Supplementary Appendix, available with the full text of this letter at NEJM.org). In an analysis in which a Cox proportional-hazards model that adjusted for age, sex, and vaccination status was used, the number of days from an initial positive polymerase-chain-reaction (PCR) assay to a negative PCR assay (adjusted hazard ratio, 0.61; 95% confidence interval [CI], 0.33 to 1.15) and the number of days from an initial positive PCR assay to culture conversion (adjusted hazard ratio, 0.77; 95% CI, 0.44 to 1.37) were similar in the two variant groups (Figure 1A through 1C and S1 through S3, and Tables S3 through S5). The median time from the initial positive PCR assay to culture conversion was 4 days (interquartile range, 3 to 5) in the delta group and 5 days (interquartile range, 3 to 9) in the omicron group; the median time from symptom onset or the initial positive PCR assay, whichever was earlier, to culture conversion was 6 days (interquartile range, 4 to 7) and 8 days (interquartile range, 5 to 10), respectively. There were no appreciable between-group differences in the time to PCR conversion or culture conversion according to vaccination status, although the sample size was quite small, which led to imprecision in the estimates (Figure 1D and 1E).

In this longitudinal cohort of participants, most of whom had symptomatic, nonsevere Covid-19 infection, the viral decay kinetics were similar with omicron infection and delta infection. Although vaccination has been shown to reduce the incidence of infection and the severity of disease, we did not find large differences in the median duration of viral shedding among participants who were unvaccinated, those who were vaccinated but not boosted, and those who were vaccinated and boosted.

Our results should be interpreted within the context of a small sample size, which limits precision, and the possibility of residual confounding in comparisons according to variant, vaccination status, and the time period of infection. Although culture positivity has been proposed as a possible proxy for infectiousness,5 additional studies are needed to correlate viral-culture positivity with confirmed transmission in order to inform isolation periods. Our data suggest that some persons who are infected with the omicron and delta SARS-CoV-2 variants shed culturable virus more than 5 days after symptom onset or an initial positive test.

Julie Boucau, Ph.D.
Caitlin Marino, B.S.
Ragon Institute, Cambridge, MA

James Regan, B.S.
Brigham and Women’s Hospital, Boston, MA

Rockib Uddin, B.S.
Massachusetts General Hospital, Boston, MA

Manish C. Choudhary, Ph.D.
James P. Flynn, B.S.
Brigham and Women’s Hospital, Boston, MA

Geoffrey Chen, B.A.
Ashley M. Stuckwisch, B.S.
Josh Mathews, A.B.
May Y. Liew, B.A.
Arshdeep Singh, B.S.
Taryn Lipiner, M.P.H.
Massachusetts General Hospital, Boston, MA

Autumn Kittilson, B.S.
Meghan Melberg, B.S.
Yijia Li, M.D.
Brigham and Women’s Hospital, Boston, MA

Rebecca F. Gilbert, B.A.
Zahra Reynolds, M.P.H.
Surabhi L. Iyer, B.A.
Grace C. Chamberlin, B.A.
Tammy D. Vyas, B.S.
Marcia B. Goldberg, M.D.
Jatin M. Vyas, M.D., Ph.D.
Massachusetts General Hospital, Boston, MA

Jonathan Z. Li, M.D.
Brigham and Women’s Hospital, Boston, MA

Jacob E. Lemieux, M.D., D.Phil.
Mark J. Siedner, M.D., M.P.H.
Amy K. Barczak, M.D.
Massachusetts General Hospital, Boston, MA

Supported by grants (to Drs. Goldberg, J.Z. Li, Lemieux, Siedner, and Barczak) from the Massachusetts Consortium for Pathogen Readiness, a grant (to Dr. Vyas) from the Massachusetts General Hospital Department of Medicine, and a grant (P30 AI060354, to the BSL3 laboratory where viral culture work was performed) from the Harvard University Center for Acquired Immunodeficiency Syndrome Research.

Disclosure forms provided by the authors are available with the full text of this letter at NEJM.org.

This letter was published on June 29, 2022, at NEJM.org.

Drs. J.Z. Li, Lemieux, Siedner, and Barczak contributed equally to this letter.